glyQ [2019-11-21 17:20:40]

glycyl-tRNA synthetase (alpha subunit)

locus: BSU_25270

pI: 4.80

mw: 33.83 kDa

protein length: 295 aa

gene length: 888 bp

function: translation

product: glycyl-tRNA synthetase (alpha subunit)

essential: yes

ec: 6.1.1.14

synonyms: yqfJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,607,762 → 2,608,649

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + glycine + tRNA^Gly --> AMP + diphosphate + glycyl-tRNA^Gly (according to UniProt)

Protein family

• [SW|Class-II aminoacyl-tRNA synthetase family] (according to UniProt)

Structure

• [PDB|1J5W] (from ''Thermotoga maritima'', 55% identity, 72% similarity)
• [PDB|6POM] (the [gene|FD84CF3E05B81BD38D8C8FF5B04728FB3912D9B5|glyQ] [SW|riboswitch] in complex with tRNA-Gly) [pubmed|31740854]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|FD84CF3E05B81BD38D8C8FF5B04728FB3912D9B5|glyQ]-[gene|337BF226112A48C39A24E6D5C7A63F864502914C|glyS]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [regulon|T-box|T-box]: anti-termination, [Pubmed|19258532,28531275], in [regulon|T-box|T-box]

  regulation

  • induced by glycine limitation ([protein|search|T-box]) [Pubmed|19258532,28531275]

References

Reviews

• 19258532

The [gene|FD84CF3E05B81BD38D8C8FF5B04728FB3912D9B5|glyQ] [SW|riboswitch]

• 15890195,15663928,15292140,16741230,12923262,24954903,26229106,23589841,28621923,30251626,31740854

Other publications

• 15378759